DR LEE KHAI WOOI (JASON)

Dr Lee Khai Wooi (Jason)

Lecturer

Ph.D in Nanobiotechnology (University Putra Malaysia),
B.Sc. (Hons) Microbiology (University Putra Malaysia).

khaiwooi.lee@taylors.edu.my
Expertise
  • Nanoparticles for pharmaceutical applications
  • Genetic engineering and molecular virology
  • Protein expression, purification and characterization
  • Drugs and gene delivery using virus-like particles and nanoparticles
  • Phage display and biopanning

Research Interests
  • Application of virus-like particle/nanoparticles for drug and gene delivery
  • Antigen display and vaccine development

Research Grants Awarded
  • Synthesis and characterization of nanoparticles from plant viruses for drug and gene delivery. MOHE FRGS (2013-2015)
  • Display of the conserved antigenic epitopes of influenza virus and cell targeting peptide on HBV nanoparticles. Taylor’s University TRGS (2013-2015)

Publications
Peer Reviewed Papers (10 most significant)
  • Lee, K. W. and Tan, W. S., 2008. Recombinant hepatitis B virus core particles: Association, dissociation and encapsidation of green fluorescent protein. J. Virol. Methods 151. 172-180. (Impact factor: 1.933, 2007).
  • Lee, K. W., Tey, B. T., Ho, K. L., Tan, W. S., 2012. Delivery of chimeric hepatitis B core particles into liver cells. J. Appl. Microbiol. 112. 119-131. (Impact factor: 2.337, 2011).
  • Lee, K. W., Tey, B. T., Ho, K. L., Tejo, B. A., 2012. Nano-glue: An alternative way to display cell-internalizing peptide (CIP) at the spikes of hepatitis B virus core nanoparticles for cell-targeting delivery. Mol. Pharm. 9, 2415-2423. (Impact factor: 5.4, 2010).
  • Yoon, K. Y., Tan, W. S., Tey, B. T., Lee, K. W., Ho, K. L., 2013. Native agarose gel electrophoresis and electroelution: A fast and cost effective method to separate the small and large Hepatitis B nucleocapsids. Electrophoresis 34, 244-253. (Impact factor: 3.303, 2011).
  • Lee, K. W., Tey, B. T., Ho, K. L., Tejo, B. A., 2013. Targeting HeLa cells using hepatitis B virus-like particle (HBVLP) decorated with nanoglue-cell-internalizing peptides. Eur. J. Pharm. Sci. 50 (Supplement 1), 11-12. (Impact factor: 2.987, 2012)

Conference Abstracts/ Proceedings (10 most significant):
  • Lee, K. W., Ong, S. T., Ng, M. Y. T., Tan, W. S., 2007. The Changes of HBV Capsid Morphology against Different Chaotropic Agents and Heat. In A Nanoscience Approach in Commercialization: 6th ASEAN Microscopy Conference. Cherating, Malaysia.
  • Lee, K. W., Tan, W. S., 2008. Dynamic Light Scattering Analysis of the Association and Dissociation of Hepatitis B Virus Core Antigen. In Microbes: Biotechnology Engine for Health and Wealth Creation: 30th Symposium of Malaysian Society for Microbiology. Kuantan, Malaysia.
  • Lee, K. W. and Tan, W. S., 2008. Recombinant hepatitis B virus core particles: Association, dissociation and encapsidation of green fluorescent protein. In Invention, Research and Innovation Exhibition 2008 (PRPI’08), Universiti Putra Malaysia, Selangor, Malaysia.
  • Lee, K. W., Tey, B. T., Tan, W. S., 2009. Hepatitis B Capsid as a Delivery Vehicle into Human Liver Cells. In Biotech Colloquium. UPM Selangor, Malaysia.
  • Yoon, K. Y., Lee, K. W., Tan, W. S., Tey, B. T., Ho, K. L., 2010. A simple method to separate large and small particles of hepatitis B virus capsids. In 35th Annual Conference of the Malaysian Society for Biochemistry & Molecular Biology. Selangor, Malaysia.
  • Lee, K. W., Tan, W. S., Tey, B. T., Ho, K. L., 2011.Hepatitis B Virus Capsid as a Cell Targeting Delivery System. In 25th Scientific Meeting of the Malaysian Society of Pharmacology and Physiology. Selangor, Malaysia.
  • Lee, K. W., Tey, B. T., Ho, K. L., Tejo, B. A., 2013. Targeting HeLa cells using hepatitis B virus-like particle (HBVLP) decorated with nanoglue-cell-internalizing peptides. In 5th BBBB International Conference, from drug discovery and formulation strategies to pharmacokinetics-pharmacodynamics, Athens, Greece.
  • Tan, F. H., Kong, J. C., Ng, J. F., Alitheen, N. B., Lee, K. W., 2016. Virus-like nanoparticles of turnip yellow mosaic virus produced in Escherichia coli: Expression, purification and stability characterization. In 4th International Congress on Nanoscience & Nanotechnology, Kuala Lumpur, Malaysia.
  • Kong, J. C., Tan, F. H., Ng, J. F., Alitheen, N. B., Lee, K. W., 2016. Turnip yellow mosaic virus (TYMV) capsid as potential molecular nanocarrier: Production and characterization of surface chemistry. In 4th International Congress on Nanoscience & Nanotechnology, Kuala Lumpur, Malaysia.

Scholarly Activities/ Industry Experience (10 most significant):
  • Adviser, SBS Outreach program (2013-2015)
  • Adviser, SBS Biosciences club (2013-2015)
  • Leader of infectious disease cluster (2013)
  • Member, SBS Entrepreneurship committee (2013-present)
  • Member, SBS Research & development committee (2015-present)
  • Leader (Social committee), International Functional Food Conference, 2013
  • Leader (Technical committee), International Functional Food Conference, 2017
  • Seminar conducted:
    • Invited speaker, “Virus-like particle (VLP)-A platform for peptide presentation via genetic & chemical modifications.” RMIT University, Melbourne, Australia, 21st March 2014.
  • Invited professional presentation/ seminar/ conference/ forum:
    • Bio-forum/Dialog:Kolaborasi antara industry-universiti-institusi penyelidikan menuju ke arah pembangunan ubatan “Biologik” di Malaysia. 15th October 2014, Inno Biologics Sdn. Bhd., Nilai, Seremban.
    • Keynote speaker, “Molecular display on virus/virus-like nanomaterials via chemical approach.” In 1st International conference on applied chemistry (ICAC-1), Hurghada, Egypt, 14-17th March, 2016.

Awards & Honours
  • Gold Medal Award-The invention/innovation of “Recombinant hepatitis B virus core particles: Association, dissociation and encapsidation of green fluorescent protein.” at the Invention, Research and Innovation Exhibition 2008 (PRPI’08).
  • Employee Education Subsidy Scheme for Postgraduate Certificate in Higher Education Teaching and Learning, 2012-2014 (Taylor’s University, Malaysia).

Professional Affiliations
  • Member, Malaysian Society for Biochemistry & Molecular Biology (2012-present)
  • Member, Asian Federation of Biotechnology (2014-present)